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Search results: Siglec-3

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Western blot analysis of CD99 expression in HUVEC cell lysate (M01724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 monoclonal antibody (Catalog # M01724-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99
Product group Antibodies
Boster Bio
Anti-CD99 Rabbit Monoclonal AntibodyM01724-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD99
  • SizePrice
Western blot analysis of CES1 expression in U937 cell lysate using anti-CES1 antibody (M01741). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # M01741) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CES1.
Product group Antibodies
Boster Bio
Anti-Liver Carboxylesterase 1 Monoclonal AntibodyM01741
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCES1
  • SizePrice
Western blot analysis of Cyclin D3 expression in K562 cell lysate (M01744). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND3 monoclonal antibody (Catalog # M01744) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCND3
Product group Antibodies
Boster Bio
Anti-Cyclin D3 CCND3 Rabbit Monoclonal AntibodyM01744
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetCCND3
  • SizePrice
Product group Antibodies
Boster Bio
Anti-CCR3 Rabbit Monoclonal AntibodyM01748-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human epidemal cells of skin, using CCR3 Antibody.
Product group Antibodies
Boster Bio
Anti-CCR3 Rabbit Monoclonal AntibodyM01748
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR3
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Calpain 1 CAPN1 Rabbit Monoclonal AntibodyM01943-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCAPN1
  • SizePrice
Western blot analysis of Calpain 1 expression in Human fetal kidney lysate.
Product group Antibodies
Boster Bio
Anti-Calpain 1 CAPN1 Rabbit Monoclonal AntibodyM01943
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCAPN1
  • SizePrice
Western blot analysis of PD-L1 (CD274) expression in Ramos cell lysate.
Product group Antibodies
Boster Bio
Anti-PD-L1 (CD274) Rabbit Monoclonal AntibodyM00109
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCD274
  • SizePrice
Western blot analysis of CDC42 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate (M00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # M00119) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
Product group Antibodies
Boster Bio
Anti-CDC42 Rabbit Monoclonal AntibodyM00119
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC42
  • SizePrice
Western blot analysis of CTCF expression in (1) HeLa cell lysate; (2) Mouse brain lysate (M00132). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTCF monoclonal antibody (Catalog # M00132) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTCF
Product group Antibodies
Boster Bio
Anti-CTCF Rabbit Monoclonal AntibodyM00132
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTCF
  • SizePrice
Western blot analysis of CD14 expression in Human tonsil cell lysate (M00137). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 monoclonal antibody (Catalog # M00137) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14
Product group Antibodies
Boster Bio
Anti-CD14 Rabbit Monoclonal AntibodyM00137
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD14
  • SizePrice
Anti-p21 CDKN1A Rabbit Monoclonal Antibody
Product group Antibodies
References
Boster Bio
Anti-p21 CDKN1A Rabbit Monoclonal AntibodyM00145-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCDKN1A
  • SizePrice
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