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ProductsBack/Anti-p21 CDKN1A Rabbit Monoclonal Antibody
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.

Anti-p21 CDKN1A Rabbit Monoclonal Antibody

M00145-2
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCDKN1A
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-p21 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EAI-3
  • Gene ID1026
  • Target name
    CDKN1A
  • Target description
    cyclin dependent kinase inhibitor 1A
  • Target synonyms
    CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDK-interaction protein 1, DNA synthesis inhibitor, cyclin-dependent kinase inhibitor 1A (p21, Cip1), melanoma differentiation associated protein 6, wild-type p53-activated fragment 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38936
  • Protein Name
    Cyclin-dependent kinase inhibitor 1
  • Scientific Description
    Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-2. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol.
    Read more
  • Ketorolac tromethamine represses senescence in aging articular chondrocytes.
    Read more
  • Intraperitoneal injection of Desferal® alleviated the age-related bone loss and senescence of bone marrow stromal cells in rats.
    Read more
  • Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy.
    Read more
  • Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles.
    Read more
  • Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.
    Read more
  • Long noncoding RNA Neat1 modulates myogenesis by recruiting Ezh2. Wang S et al., 2019 Jun 26, Cell Death Dis
    Read more
  • Down-regulation of long noncoding RNA DLX6-AS1 defines good prognosis and inhibits proliferation and metastasis in human epithelial ovarian cancer cells via Notch signaling pathway.
    Read more
  • RAP-8 ameliorates liver fibrosis by modulating cell cycle and oxidative stress.
    Read more
  • Cisatracurium Retards Cell Migration and Invasion Upon Upregulation of p53 and Inhibits the Aggressiveness of Colorectal Cancer. Yabasin IB et al., 2018, Front Physiol
    Read more

Datasheet

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MSDS

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Figure 1. Western blot analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (Catalog # A00145-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.
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