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Search results: Siglec-3

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Western blot analysis of CD38 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-CD38 Rabbit Monoclonal AntibodyM00193
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCD38
  • SizePrice
Western blot analysis of CD80 expression in Raji cell lysate.
Product group Antibodies
Boster Bio
Anti-CD80/B7 1 Rabbit Monoclonal AntibodyM00196
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCD80
  • SizePrice
Western blot analysis of CDK1 in HeLa cell lysate (M00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCDK1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human colon, using CDK1 Antibody(M00209-2) CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (M00209-2)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDK1
  • SizePrice
Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-3) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCDK1
  • SizePrice
Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (M00209-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
Product group Antibodies
Boster Bio
Anti-CDK1/Cdc2 Rabbit Monoclonal AntibodyM00209-4
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDK1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (M00220-1) CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (M00220-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-CD86/B7 2 Rabbit Monoclonal AntibodyM00220-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCD86
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human liver, using C Reactive Protein Antibody.
Product group Antibodies
Boster Bio
Anti-C Reactive Protein CRP Rabbit Monoclonal AntibodyM00249
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCRP
  • SizePrice
Western blot analysis of Chk2 expression in (1) HeLa cell lysate; (2) 293T cell lysate (M00277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 monoclonal antibody (Catalog # M00277) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2
Product group Antibodies
Boster Bio
Anti-Chk2 CHEK2 Rabbit Monoclonal AntibodyM00277
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCHEK2
  • SizePrice
Western blot analysis of IP10 expression in IP10 recombinant protein lysate.
Product group Antibodies
Boster Bio
Anti-IP10 CXCL10 Rabbit Monoclonal AntibodyM00278
ApplicationsWestern Blot
ReactivityHuman
TargetCXCL10
  • SizePrice
Western blot analysis of CSF3R expression in Caco-2 cell lysate using anti-CSF3R antibody (M00281). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF3R antigen affinity purified polyclonal antibody (Catalog # M00281) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CSF3R.
Product group Antibodies
Boster Bio
Anti-GCSF Receptor (CD114) Monoclonal AntibodyM00281
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetCSF3R
  • SizePrice
Western blot analysis of CXCR3 expression in K562 cell lysate using anti-CXCR3 antibody (M00295). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR3 antigen affinity purified polyclonal antibody (Catalog # M00295) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CXCR3.
Product group Antibodies
Boster Bio
Anti-CXCR3 Monoclonal AntibodyM00295
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCXCR3
  • SizePrice
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