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Search results: Siglec-3

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Western blot analysis of Oligodendrocyte Specific Protein expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetCLDN11
  • SizePrice
Western blot analysis of CD53 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD53
  • SizePrice
Western blot analysis of CD53 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD53
  • SizePrice
Western blot analysis of K Cadherin expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCDH6
  • SizePrice
Western blot analysis of K Cadherin expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCDH6
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCDH17
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCDH17
  • SizePrice
Western blot analysis of RAIDD expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCRADD
  • SizePrice
Western blot analysis of RAIDD expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCRADD
  • SizePrice
Figure 1. Western blot analysis of Calmodulin using anti-Calmodulin antibody (M06609). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmodulin antigen affinity purified monoclonal antibody (Catalog # M06609) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calmodulin at approximately 17 kDa. The expected band size for Calmodulin is at 17 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCALM1
  • SizePrice
AKR mouse splenocytes were stained with Mouse Anti-Mouse I-Ek-FITC (SB Cat. No. 1898-02) and Rat Anti-Mouse CD3epsilon-PE (SB Cat. No. 1535-09).
Product group Antibodies
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityMouse
TargetH2-Eb1
  • SizePrice
Product group Antibodies
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
ReactivityMouse
TargetH2-Eb1
  • SizePrice