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Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-CARRIER-FREE
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-CY3
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-DYLIGHT488
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-DYLIGHT550
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-DYLIGHT594
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-FITC
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-HRP
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-IFLUOR647
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)PA1474-PE
ApplicationsWestern Blot
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)RP1026-BIOTIN
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)RP1026-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
Anti-survivin/BIRC5 Antibody Picoband(r)RP1026-CY3
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
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