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Figure 1. Western Blotting result Western Blot of 293 cell lysate, using Anti-Akt1 RM252 at a 1:1000 dilution, showed Akt1 (~56 kDa) expression in 293 cells.
Product group Antibodies
Boster Bio
Anti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252M00024-3
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-AKT1/2 Rabbit Monoclonal AntibodyM00024-6-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-AKT1/2 Rabbit Monoclonal AntibodyM00024-6
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Flow cytometric Analysis of Jurkat cells
Product group Antibodies
Boster Bio
Anti-AKT1 Mouse Monoclonal Antibody [Clone ID: OTI4D6]M00024
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of PKB alpha using anti-PKB alpha antibody (MA1085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PKB alpha antigen affinity purified monoclonal antibody (Catalog # MA1085) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PKB alpha at approximately 60 kDa. The expected band size for PKB alpha is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)MA1085
ApplicationsWestern Blot
ReactivityChicken, Hamster, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (P00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # P00024-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-Phospho-AKT1 (T450) Rabbit Monoclonal AntibodyP00024-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal AntibodyP00024-3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
References
Boster Bio
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal AntibodyP00024-4
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420051
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420054
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt1 in human liver cell lysate with Akt1 antibody at (A) 1 and (B) 2 ug/ml.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA306225
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band (s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9/E5]TA319564
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAKT1
  • SizePrice
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