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Flow Cytometry of Mouse anti-AKT3 antibody. Cells: SK-OV3 Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT3 antibody at 1.0 microg/mL for 20 min at 4°C.
Product group Antibodies
OriGene
AKT3 Mouse Monoclonal Antibody [Clone ID: 25F6.F6.D8]TA396848
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Flow Cytometry of Mouse anti-AKT3 antibody. Cells: SK-OV3 Cells. Stimulation: none. Primary antibody: Allophycocyanin conjugated AKT3 antibody at 1.0 microg/mL for 20 min at 4°C.
Product group Antibodies
OriGene
AKT3 Mouse Monoclonal Antibody [Clone ID: 9A5.H9.G7]TA396851
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis for AKT3 using AKT3 Antibody at 2 ug/ml dilution against 30 ug/lane of human kidney lysate.
Product group Antibodies
OriGene
AKT3 (119-136) Mouse Monoclonal Antibody [Clone ID: 66C1247.1]AM06001PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Equine, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
TargetAKT3
  • SizePrice
Western blot analysis using Akt3 antibody Cat.-No AM06129SU-N against truncated Akt3 recombinant protein (Lane 1) and human ovary carcinoma tissue lysate (Lane 2).
Product group Antibodies
OriGene
AKT3 Mouse Monoclonal Antibody [Clone ID: 3B12F3F10]AM06129SU-N
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAKT3
  • SizePrice
WB: The Cell lysate derived from HELA cell line was immuno-blotted by Rabbit anti-AKT3 antibody, at 1:500.
Product group Antibodies
OriGene
AKT3 Rabbit Polyclonal AntibodyTA354476
ApplicationsWestern Blot
ReactivityHuman
TargetAKT3
  • SizePrice
Product group Antibodies
Biorbyt
Mouse Akt2 Antibody (N-term)ORB1936303
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAkt2
  • SizePrice
Product group Antibodies
Biorbyt
Mouse Akt2 (N-term)ORB2632941
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAkt2
  • SizePrice
Product group Antibodies
Biorbyt
Mouse Akt2 AntibodyORB1268691
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAkt2
  • SizePrice
Western blot analysis of extracts from NIH-3T3 cells, untreated or treated with PDGF, using Phospho-Akt (Ser473) (left) or Akt antibody (right).
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA324781
ApplicationsDot Blot, Western Blot
ReactivityHuman
TargetAKT1
  • SizePrice
AKT1 Antibody (MS4188) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 960CT4.5.1]TA325176
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt1 phosphorylation expression in PDGF treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA325220
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 56 kDa. The expected band size for AKT1 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-AKT1 Monoclonal AntibodyM00024-1-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
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