Bio-Connect

Search results: akt

2353 to 2364 of 11.328 products
Sort by
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323432 (AKT1 Antibody) at dilution 1/15 (Original magnification: x200)
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA323433 (AKT1 Antibody) at dilution 1/25. (Original magnification: x200)
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from A549 cells, using AKT1/2/3 (Ab-315/316/312) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from A549 cells, using AKT1/3 (Ab-437/434) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (P00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # P00024-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1. Positive lysates LY401580 (100ug) and LC401580 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1. Positive lysates LY401580 (100ug) and LC401580 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1 (TA504230). Positive lysates LY401580 (100ug) and LC401580 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice