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Search results: akt

2509 to 2520 of 5.421 products
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Product group Antibodies
EXBIO
Anti-EGFR PE-Cy(tm)7T7-680
ApplicationsFlow Cytometry
ReactivityEquine, Human, Mouse, Rat
  • SizePrice
Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate
Product group Lysates / Extracts
OriGene
AKT3 (NM_005465) Human Over-expression LysateLY401675
  • SizePrice
Western blot analysis of various lysates using Pan-Akt Rabbit mAb (TA420004) at1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 10s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA420004
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (TA420035) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 1s.
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420035
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of various lysates using Phospho-Akt-T308 Rabbit mAb (TA420036) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 180s.
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420036
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of lysates from Jurkat cells
Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420069
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Immunofluorescence Microscopy of Mouse Anti-AKTpS473 antibody using STED nanoscopy to evaluate AKT activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved,unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Staining: Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKTpS473 at the cell periphery.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 17F6.B11]TA396764
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Western blot analysis of Akt in CHO-K1, Jurkat, 3T3, C6 and Hela lysates using Akt antibody.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA383691
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot (WB) analysis of akt-1 polyclonal antibody at 1:2000 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA392490
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot (WB) analysis of Akt (Phospho-Ser473) polyclonal antibody at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
Product group Antibodies
OriGene
Rabbit Polyclonal AntibodyTA392565
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot (WB) analysis of Akt (A444) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug Lane3:EC9706 whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)
Product group Antibodies
OriGene
Rabbit Polyclonal AntibodyTA392572
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot (WB) analysis of AKT (phospho-S473) polyclonal antibody at 1:2000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with λ-phosphatase whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)
Product group Antibodies
OriGene
Rabbit Polyclonal AntibodyTA392585
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
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