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Western blot (WB) analysis of AKT (phospho-T308) polyclonal antibody at 1:5000 dilution Lane1:HeLa whole cell lysate(40ug) Lane2:Hela treated with λ-phosphatase whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:BV2 whole cell lysate(40ug)
Product group Antibodies
OriGene
Rabbit Polyclonal AntibodyTA392586
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
Product group Antibodies
OriGene
Rabbit Polyclonal AntibodyTA392643
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Circular map for RC224750L1
Product group DNA / RNA / Vectors
OriGene
AKT3 (NM_181690) Human Tagged Lenti ORF CloneRC224750L1
CategoryPlasmid / vector
  • SizePrice
Circular map for RC224750L2
Product group DNA / RNA / Vectors
OriGene
AKT3 (NM_181690) Human Tagged Lenti ORF CloneRC224750L2
CategoryPlasmid / vector
  • SizePrice
Circular map for RC224750L4
Product group DNA / RNA / Vectors
OriGene
AKT3 (NM_181690) Human Tagged Lenti ORF CloneRC224750L4
CategoryPlasmid / vector
  • SizePrice
Circular map for RG232795
Product group DNA / RNA / Vectors
OriGene
AKT3 (NM_001206729) Human Tagged ORF CloneRG232795
CategoryPlasmid / vector
  • SizePrice
Circular map for RR201042
Product group DNA / RNA / Vectors
OriGene
Akt1 (NM_033230) Rat Tagged ORF CloneRR201042
CategoryPlasmid / vector
  • SizePrice
Western blot analysis of various lysates using Pan-Akt Rabbit pAb (TA373418) at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 30s.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA373418
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody [N1C3-2] (GTX112949) diluted at 1:1000.
Product group Antibodies
GeneTex
14-3-3 sigma antibody [N1C3-2]GTX112949
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetSFN
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT3 antibody (GTX113312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
AKT3 antibodyGTX113312
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT3
  • SizePrice
Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613) 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Product group Antibodies
References
GeneTex
AKT1 antibodyGTX110613
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Untreated (–) and treated (+) Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (GTX134836) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT1 (phospho Ser129) antibodyGTX134836
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetAKT1
  • SizePrice
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