Bio-Connect

AKT3 antibody

GTX113312
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAKT3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    AKT3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.15 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10000
  • Target name
    AKT3
  • Target description
    AKT serine/threonine kinase 3
  • Target synonyms
    MPPH; MPPH2; PKB gamma; PKBG; PKB-GAMMA; PRKBG; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y243
  • Protein Name
    RAC-gamma serine/threonine-protein kinase
  • Scientific Description
    The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Erythropoietin protects neuroblastoma cells against etoposide and vincristine by activating ERK and AKT pathways but has no effect in kidney cells. Vazquez-Mellado MJ et al., 2015 Sep 15, Life Sci
    Read more

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetAKT3
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, ELISA
TargetAKT3
  • SizePrice
Immunoprecipitating AKT3 in mouse brain whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA15929A0Rb (6ug) + Mouse brain whole cell lysate (500ug) Lane 3: Mouse brain whole cell lysate (10ug)
Product group Antibodies
Cusabio
AKT3 AntibodyCSB-PA15929A0RB
ApplicationsImmunoPrecipitation, ELISA
TargetAKT3
  • SizePrice
Product group Antibodies
Assay Genie
TargetAKT3
  • SizePrice
WB analysis of human kidney tissue lysate using GTX13919 AKT3 antibody [66C1247]. Dilution : 2 microg/ml Loading : 30microg
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetAKT3
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetAKT3
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetAKT3
  • SizePrice
Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-AKT3 Antibody Picoband(r)A00520-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetAKT3
  • SizePrice
Anti-AKT3 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice