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Search results: akt

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Western blot analysis of extracts from NIH/3T3 cells, treated with Serum (10%, 30mins), using Akt (Ab-450) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from NIH/3T3cells, treated with PDGF (50mg /ml, 20mins), using Akt (Ab-124) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from HuvEc cells, treated with Serum (30%, 30mins), using Akt (Ab-129) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using Akt (Ab-326) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-AKT pS473 antibody. Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane. Primary antibody: AKT pS473 antibody at 1:200 dilution for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~56 kDa for AKT pS473. Other band (s): unspecific.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band (s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-Akt phospho T308 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 un-active recombinant protein. Load: 25 ng per lane. Primary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho T308. Other band (s): none
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
WB of Mouse Anti-AKT pS473 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane. Primary antibody: AKT pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:20,000 dilution.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: none. Load: 25 ng per lane. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho S473. Other band (s): none
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of AKT in 3T3, C6 lysates using AKT antibody.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of AKT in 3T3, rat heart lysates using AKT antibody.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Anti-AKT3 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice