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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
AKT antibody [N3C2], InternalGTX121937
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityFish, Human, Mouse, Porcine, Rat
TargetAKT1
  • SizePrice
AKT (phospho Ser473) antibody detects AKT (phospho Ser473) protein by immunofluorescent analysis. Sample: NIH3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AKT (phospho Ser473) stained by AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibodyGTX128414
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Porcine, Rat
TargetAKT1
  • SizePrice
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX132615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibodyGTX132615
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (GTX133175) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Thr450) antibodyGTX133175
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.
Product group Antibodies
References
GeneTex
AKT (phospho Thr308) antibodyGTX28933
ApplicationsDot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAKT1
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Product group Antibodies
GeneTex
AKT pathway Antibody PanelGTX300089
  • SizePrice
Product group Chemicals
Biosynth
Akt/SKG Substrate PeptideCRB1000597
Molecular Weight817.5 g/mol
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Chemical Structure
Product group Chemicals
SYNkinase
Akt-I-1 [473382-39-7] [473382-39-7]SYN-1005
CAS Number473382-39-7
Estimated Purity>95%
Molecular Weight378.5
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Product group Assays
Assay Genie
Rat Protein Kinase B/PKB/AKT ELISA KitRTFI01064
Assay Sample TypeSerum, Plasma
ReactivityRat
  • SizePrice
Product group Antibodies
Bioss
AKTBSM-63324R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Chemicals
Biosynth
Akt-I-1,2 HCl [473382-50-2]YTA38250
CAS Number473382-50-2
Molecular Weight339.43 g/mol
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Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody [HL2871] (GTX640148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibody [HL2871]GTX640148
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
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