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Western Blot analysis of COLO205 cells using Sec23B Polyclonal Antibody
Western Blot analysis of COLO205 cells using Sec23B Polyclonal Antibody
Western Blot analysis of COLO205 cells using Sec23B Polyclonal Antibody

SEC23B Antibody

CSB-PA006552
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSEC23B
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEC23B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10483
  • Target name
    SEC23B
  • Target description
    SEC23 homolog B, COPII component
  • Target synonyms
    CDA-II, CDAII, CDAN2, CWS7, HEMPAS, hSec23B, protein transport protein Sec23B, SEC23 homolog B, COPII coat complex component, SEC23 homolog B, coat complex II component, SEC23-like protein B, SEC23-related protein B, transport protein SEC23B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15437
  • Protein Name
    Protein transport protein Sec23B
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEC23B using anti-SEC23B antibody (A05847-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23B antigen affinity purified polyclonal antibody (Catalog # A05847-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23B at approximately 86KD. The expected band size for SEC23B is at 86KD.
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