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SEC23B Antibody

LS-C750102
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSEC23B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEC23B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 86kDa, while the observed MW by Western blot was 86kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 86kDa, while the observed MW by Western blot was 86kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10483
  • Target name
    SEC23B
  • Target description
    SEC23 homolog B, COPII component
  • Target synonyms
    CDA-II, CDAII, CDAN2, CWS7, HEMPAS, hSec23B, protein transport protein Sec23B, SEC23 homolog B, COPII coat complex component, SEC23 homolog B, coat complex II component, SEC23-like protein B, SEC23-related protein B, transport protein SEC23B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC23B using anti-SEC23B antibody (A05847-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat stomach tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23B antigen affinity purified polyclonal antibody (Catalog # A05847-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23B at approximately 86KD. The expected band size for SEC23B is at 86KD.
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