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Dot Blot of Donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityGoat
  • SizePrice
ELISA results of purified Donkey anti-Goat IgG antibody Biotin conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102-0010). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityGoat
  • SizePrice
Western blot of Phycoerythrin conjugated Donkey Anti-Goat IgG Secondary antibody.
Lane 1: Goat IgG.
Lane 2: None.
Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT.
Blocking: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.
Other band(s): None.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
  • SizePrice
Dot Blot of Donkey anti-Goat IgG Antibody Texas Red Conjugated. Antigen: Goat IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Donkey anti-Goat IgG Antibody Texas Red Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 60 min at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Chicken anti-Goat IgG Antibody Fluorescein Conjugated. Antigen: Goat IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng.
Primary antibody: n/a.
Secondary antibody: Chicken anti-Goat IgG Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityGoat
  • SizePrice
Product group Antibodies
OriGene
ApplicationsELISA
  • SizePrice
ELISA Results of Chicken Anti-Goat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution of Goat IgG Alk Phos is 1:3700. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP stabilizer (p/n MB-067) and NPP Buffer (p/n NPP-500).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice

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