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ELISA results of purified Goat anti-Human IgG F(c) antibody (min x Mouse serum proteins) tested against purified Human IgG F(c) (p/n 009-0103). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) as well as Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n 605-703-125) and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Dot Blot of Goat Anti-HUMAN IgG F(c) Fluorescein Conjugated Antibody.
Lane 1: 100ng.
Lane 2: 33.3ng.
Lane 3: 11.1ng.
Lane 4: 3.7ng.
Lane 5: 1.23ng.
Secondary Antibody: Goat Anti-Human F(c) FITC 1µg/mL.
Blocking Buffer: BlockOut MB-073 for 30 min at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
  • SizePrice
ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103) [Blue Line] and Human IgG (p/n 009-0102) [Purple Line]. The working dilution is 1:33,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Alkaline Phosphatase Conjugated (Min X Mouse serum Proteins) tested against purified Human IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The working dilution is 1:5200. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10), NPP Working Buffer (p/n NPP-B500).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Goat anti-Human IgG F(c) Antibody Biotin conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated (p/n S000-03) and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
ELISA Results of Goat Anti-Human IgG F(c) Antibody tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103) [Red Line], Human IgG Fab (p/n 009-0105) [Green Line], Human IgG (p/n 009-0102) [Blue Line]. The working dilution is 1:143,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice

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