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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified Goat anti-Human IgG Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Western Blot of Goat anti-HUMAN λ (lambda chain) Peroxidase Conjugated antibody. Lane 1: Human Lambda. Lane 2: None. Load: 50 ng per lane.
Primary Antibody: None.
Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 28 kDa for Human Lambda. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Goat anti-Human IgG Pre-Adsorbed Peroxidase Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): Human IgG splice variants and isoforms.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Western Blot of Goat anti-Human IgG Pre-Adsorbed Peroxidase Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): Human IgG splice variants and isoforms.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Western Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane.
Primary antibody: none.
Secondary antibody: Alkaline Phosphatase Human secondary antibody at 1:1,000 o/n at 4°C.
Block: MB-070 for 30 min at RT.
Reaction visualized using alkaline phosphatase for 30 seconds at RT.
Predicted/Observed size: 50kDa, 25kDa for Human IgG.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Human IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The working dilution is 1:121,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Conjugate Buffer (p/n NPP-10), NPP Working Buffer (p/n NPP-B500), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice

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