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ELISA of purified Mouse Anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG HRP. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:28,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Sheep Anti-Human IgG Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:60000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Sheep IgG HRP conjugated (p/n 613-703-168) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified Sheep Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Dot Blot of Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: none.
Secondary antibody: Sheep anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 60 min at RT.
Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
ELISA results of purified Sheep Anti-HUMAN IgG Biotin Conjugated Antibody tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of human IgG (green line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10,000 and TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice

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