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ELISA Results of purified Goat Anti-Human IgG Antibody Biotin Conjugated (Min X Mouse Serum Proteins) tested against purified Human IgG Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:175,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Biotin Conjugated Pre-AdsorbedTA397764
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Texas Red(tm) ConjugatedTA397765
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human IgG F(c) Antibody Texas Red(tm) ConjugatedTA397766
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human IgG F(ab)2 Antibody Texas Red(tm) ConjugatedTA397767
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human IgA (alpha chain) Antibody Texas Red(tm) ConjugatedTA397768
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human (kappa chain) Antibody Texas Red(tm) ConjugatedTA397769
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human (lambda chain) Antibody Texas Red(tm) ConjugatedTA397770
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human IgG (gamma chain) Antibody Texas Red(tm) ConjugatedTA397771
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Texas Red(tm) Conjugated Pre-AdsorbedTA397772
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Dot Blot of Mouse Anti-Human IgG Rhodamine Conjugated Secondary Antibody. Antigen: Human IgG. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Rhodamine mouse secondary antibody at 1:1000 for 1H at RT. Block: MB-070 for 1 H at RT.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Rhodamine ConjugatedTA397773
ApplicationsImmunoFluorescence
ReactivityHuman
  • SizePrice
Western Blot of Human IgG Antibody. M: 3 µL Opal Prestained Ladder (MB-210-0500). Lane 1: Human IgG (p/n 009-0102). Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40,000 for 30 minutes at RT. Block: (p/n MB-070) for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.
Product group Antibodies
OriGene
Human IgG (H&L) AntibodyTA397774
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Product group Antibodies
OriGene
Human IgG (H&L) Antibody Fluorescein ConjugatedTA397775
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityHuman
  • SizePrice
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