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Secondary

1033 to 1044 of 3.085 products
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Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Human IgA Antibody Alkaline Phosphatase Conjugated.
Antigen: Human IgA.
Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng.
Primary antibody: n/a
Secondary antibody: Human IgA Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 60 min at RT.
Visualized using Alkaline Phosphatase Substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Alkaline Phosphatase Conjugated Goat anti-Human IgG Gamma Chain antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane.
Primary antibody: none.
Secondary antibody: Alkaline Phosphatase human secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min RT.
Predicted/Observed size: 55 kDa for Human IgG Gamma Chain. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Human IgG.
Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
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ELISA results of purified Goat Anti-Human IgG Biotin Conjugated Antibody tested against purified human IgG. Each well was coated in duplicate with 1.0 µg of human IgG (purple line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10,000, and TMB-8000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA of purified Goat Anti-Human IgG F(ab')2 Antibody Biotin Conjugated tested against purified Human IgG F(ab')2 Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab')2 (p/n 009-0104). The working dilution is 1:108,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western blot of Biotin conjugated Goat Anti-Human κ secondary antibody.
Lane 1: Human κ.
Lane 2: Human λ.
Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT.
Blocking: MB-070 for 30 min at RT.
Predicted/Observed size: 28 kDa, 28 kDa for Human κ.
Other band(s): None.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice

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