IHC-P analysis of human breast tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human colon tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_2389_IHC-P_w_23061420_197.webp "IHC-P analysis of human colon tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_2390_IHC-P_w_23061420_439.webp "IHC-P analysis of human kidney tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lung tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_2391_IHC-P_w_23061420_123.webp "IHC-P analysis of human lung tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84161 SEK1 / MKK4 antibody [4B4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_314_FACS_w_23061420_747.webp "FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84161 SEK1 / MKK4 antibody [4B4].")
![IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84161 SEK1 / MKK4 antibody [4B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_3372_IP_w_23061420_754.webp "IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84161 SEK1 / MKK4 antibody [4B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84161 SEK1 / MKK4 antibody [4B4]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_4163_WB_w_23061420_977.webp "WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84161 SEK1 / MKK4 antibody [4B4]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_970_ICCIF_w_23061420_186.webp "ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].")
IHC-P analysis of human breast tissue using GTX84161 SEK1 / MKK4 antibody [4B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
SEK1 / MKK4 antibody [4B4]
GTX84161
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMAP2K4
Overview
- SupplierGeneTex
- Product NameSEK1 / MKK4 antibody [4B4]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:150. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B4
- Concentration1.09 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsc-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; MKK4; PRKMK4; SAPK/ERK kinase 1; SAPKK1; SAPKK-1; SEK1; SERK1; SKK1; stress-activated protein kinase kinase 1
- HostMouse
- IsotypeIgG2a
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203