IHC-P analysis of human kidney carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human thyroid carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_2393_IHC-P_w_23061420_567.webp "IHC-P analysis of human thyroid carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lung tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_2394_IHC-P_w_23061420_319.webp "IHC-P analysis of human lung tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_2395_IHC-P_w_23061420_411.webp "IHC-P analysis of human pancreas tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human thyroid tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_2396_IHC-P_w_23061420_641.webp "IHC-P analysis of human thyroid tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84162 SEK1 / MKK4 antibody [8A8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_315_FACS_w_23061420_134.webp "FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84162 SEK1 / MKK4 antibody [8A8].")
![IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84162 SEK1 / MKK4 antibody [8A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_3373_IP_w_23061420_736.webp "IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84162 SEK1 / MKK4 antibody [8A8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_4164_WB_w_23061420_636.webp "WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_4166_WB_w_23061420_434.webp "WB analysis of various cell lines using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 35 ug per lane")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_4449_WB_w_23061420_553.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 10 ug per lane Dilution : 1:500")
IHC-P analysis of human kidney carcinoma tissue using GTX84162 SEK1 / MKK4 antibody [8A8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
SEK1 / MKK4 antibody [8A8]
GTX84162
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAP2K4
Overview
- SupplierGeneTex
- Product NameSEK1 / MKK4 antibody [8A8] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:150. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8A8
- Concentration2.36 mg/ml
- ConjugateUnconjugated
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsJNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
- HostMouse
- IsotypeIgG2b
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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