
IHC-P analysis of human breast carcinoma tissue using GTX78948 SEK1 / MKK4 (phospho Thr261) antibody.
SEK1 / MKK4 (phospho Thr261) antibody
GTX78948
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMAP2K4
Overview
- SupplierGeneTex
- Product NameSEK1 / MKK4 (phospho Thr261) antibody
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsJNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
- HostRabbit
- IsotypeIgG
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionThis gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![WB analysis of HepG2 (1), K562 (2), and HEK293 (3) cell lysate using GTX60399 SEK1 / MKK4 antibody [5H4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60399/GTX60399_20170912_WB_w_23061123_426.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230414_WB_multiple_B_23041723_957.webp)
![Untransfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_T-44837_20221230_WB_shRNA_watermark_23010400_431.webp)
![ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_970_ICCIF_w_23061420_186.webp)
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_4449_WB_w_23061420_553.webp)
