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Selenophosphate synthetase 1 antibody

GTX107395
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetSEPHS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Selenophosphate synthetase 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.92 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22929
  • Target name
    SEPHS1
  • Target description
    selenophosphate synthetase 1
  • Target synonyms
    SELD; selenide, water dikinase 1; selenium donor protein 1; selenophosphate synthase 1; selenophosphate synthetase 1 +E9; selenophosphate synthetase 1 +E9a; selenophosphate synthetase 1 delta E2; selenophosphate synthetase 1 delta E8; selenophosphate synthetase 1 major; SPS; SPS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49903
  • Protein Name
    Selenide, water dikinase 1
  • Scientific Description
    This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Selenophosphate Synthetase 1/SEPHS1 using anti-Selenophosphate Synthetase 1/SEPHS1 antibody (A10102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Selenophosphate Synthetase 1/SEPHS1 antigen affinity purified polyclonal antibody (Catalog # A10102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Selenophosphate Synthetase 1/SEPHS1 at approximately 50 kDa. The expected band size for Selenophosphate Synthetase 1/SEPHS1 is at 43 kDa.
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