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Western blot All lanes: SEPHS1 antibody at 3.05ug/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Lane 4: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36 kDa Observed band size: 43 kDa
Western blot All lanes: SEPHS1 antibody at 3.05ug/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Lane 4: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36 kDa Observed band size: 43 kDa
Western blot All lanes: SEPHS1 antibody at 3.05ug/ml Lane 1: Mouse liver tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Lane 4: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 36 kDa Observed band size: 43 kDa

SEPHS1 Antibody

CSB-PA021015ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSEPHS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPHS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22929
  • Target name
    SEPHS1
  • Target description
    selenophosphate synthetase 1
  • Target synonyms
    SELD, SPS, SPS1, VERBRAS2, selenide, water dikinase 1, selenium donor protein 1, selenophosphate synthase 1, selenophosphate synthetase 1 +E9, selenophosphate synthetase 1 +E9a, selenophosphate synthetase 1 delta E2, selenophosphate synthetase 1 delta E8, selenophosphate synthetase 1 major
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49903
  • Protein Name
    Selenide, water dikinase 1
  • Scientific Description
    Synthesizes selenophosphate from selenide and ATP.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Selenophosphate Synthetase 1/SEPHS1 using anti-Selenophosphate Synthetase 1/SEPHS1 antibody (A10102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Selenophosphate Synthetase 1/SEPHS1 antigen affinity purified polyclonal antibody (Catalog # A10102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Selenophosphate Synthetase 1/SEPHS1 at approximately 50 kDa. The expected band size for Selenophosphate Synthetase 1/SEPHS1 is at 43 kDa.
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