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Western Blot analysis of MCF7 cells using Septin 7 Polyclonal Antibody
Western Blot analysis of MCF7 cells using Septin 7 Polyclonal Antibody
Western Blot analysis of MCF7 cells using Septin 7 Polyclonal Antibody

SEPT7 Antibody

CSB-PA004069
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEPTIN7
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID989
  • Target name
    SEPTIN7
  • Target description
    septin 7
  • Target synonyms
    CDC10, CDC3, NBLA02942, SEPT7, SEPT7A, Septin-7, septin-7, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16181
  • Protein Name
    Septin-7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT7/SEPTIN7 antigen affinity purified polyclonal antibody (Catalog # A30759-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT7/SEPTIN7 at approximately 51 kDa. The expected band size for SEPT7/SEPTIN7 is at 51 kDa.
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