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Confocal immunofluorescence analysis of U2OS cells using SEPT9 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using SEPT9 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using SEPT9 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

SEPT9 Polyclonal Antibody

E-AB-63893
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SEPT9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human SH-SY5Y whole cell lsates, Lane 8: huamn PC-3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSF/SEPTIN9 antigen affinity purified polyclonal antibody (Catalog # A05279-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSF/SEPTIN9 at approximately 70 kDa. The expected band size for MSF/SEPTIN9 is at 65 kDa.
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