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SERPINE1 / PAI-1 Antibody

LS-C17296
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSERPINE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINE1 / PAI-1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA and Western Blot. ELISA: 1:10000 to 1:100000. Western Blot: 1:1000 to 1:10000. Optimum dilutions to be determined by researcher.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:1000) Suitable for use in ELISA and Western Blot. ELISA: 1:10000 to 1:100000. Western Blot: 1:1000 to 1:10000. Optimum dilutions to be determined by researcher.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5054
  • Target name
    SERPINE1
  • Target description
    serpin family E member 1
  • Target synonyms
    endothelial plasminogen activator inhibitor; PAI; PAI1; PAI-1; PLANH1; plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (A00637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody (Catalog # A00637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.
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