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ProductsBack/PAI-1 antibody [GT1223]
WB analysis of HepG2 whole cell lysate using GTX02817 PAI-1 antibody [GT1223]. Dilution : 1:1000 Loading : 25microg
WB analysis of HepG2 whole cell lysate using GTX02817 PAI-1 antibody [GT1223]. Dilution : 1:1000 Loading : 25microg
WB analysis of HepG2 whole cell lysate using GTX02817 PAI-1 antibody [GT1223]. Dilution : 1:1000 Loading : 25microg

PAI-1 antibody [GT1223]

GTX02817
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSERPINE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PAI-1 antibody [GT1223] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1223
  • Concentration
    1.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5054
  • Target name
    SERPINE1
  • Target description
    serpin family E member 1
  • Target synonyms
    endothelial plasminogen activator inhibitor; PAI; PAI1; PAI-1; PLANH1; plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05121
  • Protein Name
    Plasminogen activator inhibitor 1
  • Scientific Description
    This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (A00637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody (Catalog # A00637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.
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