More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-PAI-1 [8H9D4]

Anti-PAI-1 [8H9D4]

Ab03641-1.1
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetSERPINE1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PAI-1 [8H9D4]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is reported to have strong inhibitory effect towards PAI-1 activity. The cross reactivity of this antibody towards other PAI-1 than human was done using ELISA (PMID: 9048903). This antibody shifts the reaction to the substrate pathway without decreasing the rate-limiting constant for reactive center loop insertion by tPA (PMID: 12223472). This antibody interferes with the final locking step in the serpin/proteinase interaction, thereby explaining its substrate inducing properties (PMID: 12871373).
  • Applications
    ELISA, Neutralisation/Blocking
  • Applications Supplier
    Inhibit; neutralize; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8H9D4
  • Gene ID5054
  • Target name
    SERPINE1
  • Target description
    serpin family E member 1
  • Target synonyms
    endothelial plasminogen activator inhibitor; PAI; PAI1; PAI-1; PLANH1; plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05121
  • Protein Name
    Plasminogen activator inhibitor 1
  • Reactivity Supplier
    Human, pig
  • Reactivity Supplier Note
    The original antibody was generated using PAI-1 complex as an immunogen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
PAI-1/Serpin E1 Monoclonal AntibodyCAB19096
TargetSERPINE1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAI1) Polyclonal Antibody, Unconjugated (bs-6562R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product group Antibodies
Bioss Antibodies
PAI1 Polyclonal AntibodyBS-6562R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSERPINE1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SERPINE1 Antibody144-62202
ApplicationsImmunoFluorescence, Western Blot
TargetSERPINE1
  • SizePrice
Anti-PAI1 Antibody
Product group Antibodies
Antibodies.com
Anti-PAI1 AntibodyA15108
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
Biosynth
SERPINE1 antibody70R-5427
  • SizePrice
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in endothelial cells.
Product group Antibodies
Atlas Antibodies
Anti-SERPINE1 AntibodyHPA050039
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetSERPINE1
  • SizePrice
Product group Antibodies
MedChem Express
PAI1 Antibody (YA1524, Rabbit)HY-P81779
TargetSERPINE1
  • SizePrice
Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (A00637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody (Catalog # A00637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.
Product group Antibodies
Boster Bio
Anti-PAI1/Serpine1 Antibody Picoband(r)A00637-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetSERPINE1
  • SizePrice
Western Blot analysis of Jurkat cells using PAI-1 Polyclonal Antibody
Product group Antibodies
Cusabio
SERPINE1 AntibodyCSB-PA003699
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSERPINE1
  • SizePrice
Product group Antibodies
Biomatik
Serpine1 Polyclonal AntibodyCAC09137
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetSERPINE1
  • SizePrice