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Western blot analysis using anti-3BP2 at 10 ug/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).
Western blot analysis using anti-3BP2 at 10 ug/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).
Western blot analysis using anti-3BP2 at 10 ug/ml on recombinant full lenth 3BP2 protein (1), 3BP2 protein minus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).

SH3BP2 Sheep Polyclonal Antibody

AP05082PU-N
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetSH3BP2
250 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SH3BP2 Sheep Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6452
  • Target name
    SH3BP2
  • Target description
    SH3 domain binding protein 2
  • Target synonyms
    3BP2; 3BP-2; Abl-SH3 binding protein 2; CRBM; CRPM; epididymis secretory sperm binding protein; RES4-23; SH3 domain-binding protein 2; TNFAIP3 interacting protein 2
  • Host
    Sheep
  • Isotype
    IgG
  • Scientific Description
    SH3BP2 sheep polyclonal antibody, Purified
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 3BP2/SH3BP2 using anti-3BP2/SH3BP2 antibody (A04008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-3BP2/SH3BP2 antigen affinity purified polyclonal antibody (Catalog # A04008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 3BP2/SH3BP2 at approximately 70 kDa. The expected band size for 3BP2/SH3BP2 is at 62,70 kDa.
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