FACS analysis of HeLa cells using GTX83657 SH3GL1 antibody [2F5]. Red : Primary antibody Blue : Negative control antibody
![FACS analysis of Jurkat cells using GTX83657 SH3GL1 antibody [2F5]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_106_FACS_w_23061420_483.webp "FACS analysis of Jurkat cells using GTX83657 SH3GL1 antibody [2F5]. Red : Primary antibody Blue : Negative control antibody")
![IHC-P analysis of human bladder carcinoma tissue using GTX83657 SH3GL1 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_1674_IHC-P_w_23061420_102.webp "IHC-P analysis of human bladder carcinoma tissue using GTX83657 SH3GL1 antibody [2F5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of wild type and SH3GL1 knock out 293T cell lysate(10microg per lane) using GTX83657 SH3GL1 antibody [2F5]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_3795_20181228_WB_w_23061420_621.webp "WB analysis of wild type and SH3GL1 knock out 293T cell lysate(10microg per lane) using GTX83657 SH3GL1 antibody [2F5]. Dilution : 1:500")
![WB analysis of various cell lines using GTX83657 SH3GL1 antibody [2F5]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_3797_WB_w_23061420_879.webp "WB analysis of various cell lines using GTX83657 SH3GL1 antibody [2F5]. Loading : 35 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with SH3GL1 plasmid using GTX83657 SH3GL1 antibody [2F5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_764_ICCIF_w_23061420_265.webp "ICC/IF analysis of COS7 cells transiently transfected with SH3GL1 plasmid using GTX83657 SH3GL1 antibody [2F5].")
![Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [2F5] (GTX83657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83657/GTX83657_822205142_20221202_WB_TPM_watermark_22120719_701.webp "Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [2F5] (GTX83657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
FACS analysis of HeLa cells using GTX83657 SH3GL1 antibody [2F5]. Red : Primary antibody Blue : Negative control antibody
SH3GL1 antibody [2F5]
GTX83657
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSH3GL1
Overview
- SupplierGeneTex
- Product NameSH3GL1 antibody [2F5] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2F5
- Concentration0.64 mg/ml
- ConjugateUnconjugated
- Gene ID6455
- Target nameSH3GL1
- Target descriptionSH3 domain containing GRB2 like 1, endophilin A2
- Target synonymsCNSA1; EEN; EEN fusion partner of MLL; endophilin-2; endophilin-A2; extra 11-19 leukemia fusion; extra eleven-nineteen leukemia fusion gene protein; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1; SH3-containing Grb-2-like 1 protein; SH3D2B; SH3-domain GRB2-like 1; SH3P8
- HostMouse
- IsotypeIgG1
- Protein IDQ99961
- Protein NameEndophilin-A2
- Scientific DescriptionImplicated in endocytosis. May recruit other proteins to membranes with high curvature.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203