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ProductsBack/SH3PX1 (SNX9) Mouse Monoclonal Antibody [Clone ID: OTI1E2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SNX9 (Cat# RC202822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9(Cat# TA501250). Positive lysates [LY402520] (100ug) and [LC402520] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SNX9 (Cat# RC202822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9(Cat# TA501250). Positive lysates [LY402520] (100ug) and [LC402520] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SNX9 (Cat# RC202822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9(Cat# TA501250). Positive lysates [LY402520] (100ug) and [LC402520] (20ug) can be purchased separately from OriGene.

SH3PX1 (SNX9) Mouse Monoclonal Antibody [Clone ID: OTI1E2]

CF501250
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSNX9
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SH3PX1 (SNX9) Mouse Monoclonal Antibody [Clone ID: OTI1E2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E2
  • Gene ID51429
  • Target name
    SNX9
  • Target description
    sorting nexin 9
  • Target synonyms
    SDP1, SH3PX1, SH3PXD3A, WISP, sorting nexin-9, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A, Wiskott-Aldrich syndrome protein (WASP) interactor protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9Y5X1
  • Protein Name
    Sorting nexin-9
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SNX9 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SH3PX1/SNX9 using anti-SH3PX1/SNX9 antibody (A03796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3PX1/SNX9 antigen affinity purified polyclonal antibody (Catalog # A03796-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3PX1/SNX9 at approximately 78 kDa. The expected band size for SH3PX1/SNX9 is at 67 kDa.
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