FACS analysis of HEK293T cells transfected with either SHC1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83655 SHC1 antibody [1D3].
![WB analysis of HEK293T cells transfected with SHC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83655 SHC1 antibody [1D3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83655/GTX83655_3793_WB_w_23061420_842.webp "WB analysis of HEK293T cells transfected with SHC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83655 SHC1 antibody [1D3]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with SHC1 plasmid using GTX83655 SHC1 antibody [1D3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83655/GTX83655_761_ICCIF_w_23061420_265.webp "ICC/IF analysis of COS7 cells transiently transfected with SHC1 plasmid using GTX83655 SHC1 antibody [1D3].")
![ICC/IF analysis of HT29 cells using GTX83655 SHC1 antibody [1D3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83655/GTX83655_762_ICCIF_w_23061420_426.webp "ICC/IF analysis of HT29 cells using GTX83655 SHC1 antibody [1D3].")
FACS analysis of HEK293T cells transfected with either SHC1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83655 SHC1 antibody [1D3].
SHC1 antibody [1D3]
GTX83655
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSHC1
Overview
- SupplierGeneTex
- Product NameSHC1 antibody [1D3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1D3
- Concentration0.93 mg/ml
- ConjugateUnconjugated
- Gene ID6464
- Target nameSHC1
- Target descriptionSHC adaptor protein 1
- Target synonymsSH2 domain protein C1; SHC; SHC (Src homology 2 domain containing) transforming protein 1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A
- HostMouse
- IsotypeIgG1
- Protein IDP29353
- Protein NameSHC-transforming protein 1
- Scientific DescriptionSignaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203