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ProductsBack/Sheep anti Human chk 1 kinase

Sheep anti Human chk 1 kinase

K100P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetCHEK1
250 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human chk 1 kinase
  • Delivery Days Customer
    7
  • Application Supplier Note
    Western Blot at 1 - 10 ug/ml.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    Western Blotting;Immunoprecipitation
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1111
  • Target name
    CHEK1
  • Target description
    checkpoint kinase 1
  • Target synonyms
    cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; CHK1; CHK1 checkpoint homolog; Chk1-S; serine/threonine-protein kinase Chk1
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDO14757
  • Protein Name
    Serine/threonine-protein kinase Chk1
  • Scientific Description
    sheep anti human chk 1 kinase, checkpoint kinase 1
  • Shelf life instruction
    See expiration date on vial
  • Source
    Sheep were immunized with a protein corresponding to amino acid sequence 1-474 of human chk-1, having a molecular weight of 54 kDa.
  • Reactivity Supplier
    Human;Mouse;Rat;Monkey
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Chk1/CHEK1 using anti-Chk1/CHEK1 antibody (A01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
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