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ProductsBack/Sheep anti Human PTP (HCPTP)

Sheep anti Human PTP (HCPTP)

P240P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetACP1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human PTP (HCPTP)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antbibody can be used for Western blotting and immunoprecipitation (1-10microg/ml. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID52
  • Target name
    ACP1
  • Target description
    acid phosphatase 1
  • Target synonyms
    HAAP, LMW-PTP, LMWPTP, low molecular weight phosphotyrosine protein phosphatase, LMW-PTPase, acid phosphatase 1, soluble, acid phosphatase of erythrocyte, adipocyte acid phosphatase, cytoplasmic phosphotyrosyl protein phosphatase, low molecular weight cytosolic acid phosphatase, protein tyrosine phosphatase, red cell acid phosphatase 1, testicular secretory protein Li 37
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDP24666
  • Protein Name
    Low molecular weight phosphotyrosine protein phosphatase
  • Scientific Description
    lmwPTP, sheep anti human low molecular weight protein tyrosine phosphatase, Human Red Cell Protein Tyrosine phosphatase
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human;Mouse;Rat;Bovine
  • Reactivity Supplier Note
    The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Acid phosphatase/ACP1 using anti-Acid phosphatase/ACP1 antibody (A02167-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hele whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid phosphatase/ACP1 antigen affinity purified polyclonal antibody (Catalog # A02167-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid phosphatase/ACP1 at approximately 17 kDa. The expected band size for Acid phosphatase/ACP1 is at 17 kDa.
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