More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI2F4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.

SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI2F4]

CF501759
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPTPN11
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI2F4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2F4
  • Gene ID5781
  • Target name
    PTPN11
  • Target description
    protein tyrosine phosphatase non-receptor type 11
  • Target synonyms
    BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, tyrosine-protein phosphatase non-receptor type 11, SH2 domain-containing protein tyrosine phosphatase 2, protein-tyrosine phosphatase 1D, protein-tyrosine phosphatase 2C
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ06124
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 11
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PTPN11 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
SHP2 AntibodyCAB2793
ReactivityHuman
TargetPTPN11
  • SizePrice
Western Blot analysis of 293 cells using SH-PTP2 Polyclonal Antibody
Product group Antibodies
Cusabio
PTPN11 AntibodyCSB-PA004086
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPTPN11
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-SHP2 / PTPN11EB05114
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetPTPN11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PTPN11 / SHP-2 / NS1 AntibodyLS-C404276
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPTPN11
  • SizePrice
Product group Antibodies
Biomatik
Phospho-Ptpn11 (Y542) Recombinant AntibodyCAC12481
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetPTPN11
  • SizePrice
Figure 1. Western blot analysis of SHP2 using anti-SHP2 antibody (PB9675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug, Lane 6: SW620 Whole Cell Lysate at 40ug, Lane 7: HEPG2 Whole Cell Lysate at 40ug, Lane 8: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68 kDa. The expected band size for SHP2 is at 68 kDa.
Product group Antibodies
Boster Bio
Anti-SHP2/PTPN11 Antibody Picoband(r)PB9675-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPTPN11
  • SizePrice
Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody (GTX101062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
SHP2 antibodyGTX101062
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPTPN11
  • SizePrice
Product group Antibodies
Bioss
PTPN11 Recombinant Antbody Kit (KD-Validated)BSM-90134R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPTPN11
  • SizePrice
Product group Antibodies
Biosynth
PTPN11 antibody10R-5511
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PTPN11 Antibody144-65590
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPTPN11
  • SizePrice