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ProductsBack/SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI3F2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11 (RC220029, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11.

SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI3F2]

CF501758
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetPTPN11
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SHP2 (PTPN11) Mouse Monoclonal Antibody [Clone ID: OTI3F2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F2
  • Gene ID5781
  • Target name
    PTPN11
  • Target description
    protein tyrosine phosphatase non-receptor type 11
  • Target synonyms
    BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, tyrosine-protein phosphatase non-receptor type 11, SH2 domain-containing protein tyrosine phosphatase 2, protein-tyrosine phosphatase 1D, protein-tyrosine phosphatase 2C
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ06124
  • Protein Name
    Tyrosine-protein phosphatase non-receptor type 11
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PTPN11 mouse monoclonal antibody, clone OTI3F2 (formerly 3F2)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SHP2 using anti-SHP2 antibody (PB9675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug, Lane 6: SW620 Whole Cell Lysate at 40ug, Lane 7: HEPG2 Whole Cell Lysate at 40ug, Lane 8: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68 kDa. The expected band size for SHP2 is at 68 kDa.
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