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ProductsBack/Signal Transducer And Activator Of Transcription 5A (STAT5A) Polyclonal Antibody

Signal Transducer And Activator Of Transcription 5A (STAT5A) Polyclonal Antibody

CAU25403
Biomatik
ApplicationsWestern Blot
Product group Antibodies
TargetSTAT5A
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Overview

  • Supplier
    Biomatik
  • Product Name
    Signal Transducer And Activator Of Transcription 5A (STAT5A) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6776
  • Target name
    STAT5A
  • Target description
    signal transducer and activator of transcription 5A
  • Target synonyms
    epididymis secretory sperm binding protein; MGF; signal transducer and activator of transcription 5A; STAT5
  • Host
    Rabbit
  • Protein IDP42229
  • Protein Name
    Signal transducer and activator of transcription 5A
  • Scientific Description
    The Signal Transducer And Activator Of Transcription 5A (STAT5A) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT5A/B using anti-STAT5A/B antibody (A01087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A/B antigen affinity purified polyclonal antibody (Catalog # A01087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A/B at approximately 90-95 kDa. The expected band size for STAT5A/B is at 91 kDa.
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