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Western Blot analysis of HeLa cells using Stat5 Polyclonal Antibody
Western Blot analysis of HeLa cells using Stat5 Polyclonal Antibody
Western Blot analysis of HeLa cells using Stat5 Polyclonal Antibody

STAT5A/STAT5B Antibody

CSB-PA004177
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT5A
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAT5A/STAT5B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6776
  • Target name
    STAT5A
  • Target description
    signal transducer and activator of transcription 5A
  • Target synonyms
    epididymis secretory sperm binding protein; MGF; signal transducer and activator of transcription 5A; STAT5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42229
  • Protein Name
    Signal transducer and activator of transcription 5A
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STAT5A/B using anti-STAT5A/B antibody (A01087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A/B antigen affinity purified polyclonal antibody (Catalog # A01087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A/B at approximately 90-95 kDa. The expected band size for STAT5A/B is at 91 kDa.
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