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ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. IP reaction : 1.0ul antibody / 100ul sample
ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. IP reaction : 1.0ul antibody / 100ul sample
ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes using GTX23479 SIN3A antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. IP reaction : 1.0ul antibody / 100ul sample

SIN3A antibody

GTX23479
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSIN3A
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Overview

  • Supplier
    GeneTex
  • Product Name
    SIN3A antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 microg/ml. ICC/IF: 1:50-1:500. IHC-P: 1:50-1:500. ChIP assay: 1-3 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25942
  • Target name
    SIN3A
  • Target description
    SIN3 transcription regulator family member A
  • Target synonyms
    histone deacetylase complex subunit Sin3a; paired amphipathic helix protein Sin3a; SIN3 homolog A, transcription regulator; transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; transcriptional regulator, SIN3A; WITKOS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ST3
  • Protein Name
    Paired amphipathic helix protein Sin3a
  • Scientific Description
    The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203