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SKP1 Antibody (clone 4E+11)

LS-C169443
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSKP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SKP1 Antibody (clone 4E+11)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4E+11
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6500
  • Target name
    SKP1
  • Target description
    S-phase kinase associated protein 1
  • Target synonyms
    cyclin A/CDK2-associated p19; cyclin-A/CDK2-associated protein p19; EMC19; OCP2; OCP-2; OCP-II; organ of Corti protein 2; organ of Corti protein II; p19A; p19skp1; RNA polymerase II elongation factor-like protein OCP2; SIII; SKP1A; S-phase kinase-associated protein 1; TCEB1L; transcription elongation factor B polypeptide 1-like
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SKP1 using anti-SKP1 antibody (A00476-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP1 antigen affinity purified polyclonal antibody (Catalog # A00476-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP1 at approximately 19KD. The expected band size for SKP1 is at 19KD.
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