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Western Blot Positive WB detected in: Rat heart tissue All lanes: SLC11A1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: SLC11A1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: SLC11A1 antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48 kDa Observed band size: 60 kDa

SLC11A1 Antibody

CSB-PA021380LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSLC11A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC11A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6556
  • Target name
    SLC11A1
  • Target description
    solute carrier family 11 member 1
  • Target synonyms
    LSH; natural resistance-associated macrophage protein 1; NRAMP; NRAMP 1; NRAMP1; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1; solute carrier family 11 (sodium/phosphate symporters), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49279
  • Protein Name
    Natural resistance-associated macrophage protein 1
  • Scientific Description
    Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human THP-1 whole cell lysates Lane 3: human placenta tissue lysates Lane 4: human A549 whole cell lysates Lane 5: human Caco-2 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: human U2OS whole cell lysates Lane 8: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC11A1 antigen affinity purified polyclonal antibody (Catalog # A02547-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC11A1 at approximately 90-120KD. The expected band size for SLC11A1 is at 60KD.
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