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SLC11A1 Polyclonal Antibody

CAC12925
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetSLC11A1
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Overview

  • Supplier
    Biomatik
  • Product Name
    SLC11A1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6556
  • Target name
    SLC11A1
  • Target description
    solute carrier family 11 member 1
  • Target synonyms
    LSH, NRAMP, NRAMP1, natural resistance-associated macrophage protein 1, Leishmaniasis resistance, NRAMP 1, solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1, solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1, solute carrier family 11 (sodium/phosphate symporters), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49279
  • Protein Name
    Natural resistance-associated macrophage protein 1
  • Scientific Description
    The SLC11A1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human THP-1 whole cell lysates Lane 3: human placenta tissue lysates Lane 4: human A549 whole cell lysates Lane 5: human Caco-2 whole cell lysates Lane 6: human HepG2 whole cell lysates Lane 7: human U2OS whole cell lysates Lane 8: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC11A1 antigen affinity purified polyclonal antibody (Catalog # A02547-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC11A1 at approximately 90-120KD. The expected band size for SLC11A1 is at 60KD.
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