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ProductsBack/SLC22A3 / OCT3 Antibody (aa46-95)

SLC22A3 / OCT3 Antibody (aa46-95)

LS-C40440
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSLC22A3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC22A3 / OCT3 Antibody (aa46-95)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (2 - 5 µg/ml), WB (0.2 - 1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6581
  • Target name
    SLC22A3
  • Target description
    solute carrier family 22 member 3
  • Target synonyms
    EMT, EMTH, OCT3, solute carrier family 22 member 3, EMT organic cation transporter 3, extraneuronal monoamine transporter, organic cation transporter 3, solute carrier family 22 (extraneuronal monoamine transporter), member 3, solute carrier family 22 (organic cation transporter), member 3
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (A04914-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # A04914-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD.
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