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Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using SLC22A8 Polyclonal Antibody at dilution 1:70
Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using SLC22A8 Polyclonal Antibody at dilution 1:70
Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using SLC22A8 Polyclonal Antibody at dilution 1:70

SLC22A8 Polyclonal Antibody

E-AB-16017
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SLC22A8 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA240950(SLC22A8 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/SLC22A8 antigen affinity purified polyclonal antibody (Catalog # A04087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/SLC22A8 at approximately 62 kDa. The expected band size for OAT3/SLC22A8 is at 60 kDa.
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