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Western blot analysis of extracts of various cell lines using SLC34A2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SLC34A2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SLC34A2 Polyclonal Antibody at dilution of 1:3000.

SLC34A2 Polyclonal Antibody

E-AB-65626
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SLC34A2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a pH-sensitive sodium-dependent phosphate transporter. Phosphate uptake is increased at lower pH. Defects in this gene are a cause of pulmonary alveolar microlithiasis. Three transcript variants encoding two different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC34A2 antigen affinity purified polyclonal antibody (Catalog # A03957-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC34A2 at approximately 76-130KD. The expected band size for SLC34A2 is at 76KD.
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