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Immunohistochemistry of paraffin-embedded Human renal cancer tissue using SLC9A3R2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human renal cancer tissue using SLC9A3R2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human renal cancer tissue using SLC9A3R2 Polyclonal Antibody at dilution 1:40

SLC9A3R2 Polyclonal Antibody

E-AB-15845
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SLC9A3R2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A3R2 antigen affinity purified polyclonal antibody (Catalog # A05624-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A3R2 at approximately 45 kDa. The expected band size for SLC9A3R2 is at 37 kDa.
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