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WB analysis of various sample lysates using GTX66532 SLD5 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66532 SLD5 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66532 SLD5 antibody. Dilution : 1:1000 Loading : 25microg per lane

SLD5 antibody

GTX66532
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGINS4
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLD5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84296
  • Target name
    GINS4
  • Target description
    GINS complex subunit 4
  • Target synonyms
    SLD5, DNA replication complex GINS protein SLD5, SLD5 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRT9
  • Protein Name
    DNA replication complex GINS protein SLD5
  • Scientific Description
    The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM, Mar 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLD5/GINS4 using anti-SLD5/GINS4 antibody (A10548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLD5/GINS4 antigen affinity purified polyclonal antibody (Catalog # A10548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLD5/GINS4 at approximately 28 kDa. The expected band size for SLD5/GINS4 is at 26 kDa.
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